Fig. 4.
Kinetics of LKLF and CYP1B1 differential expression as determined by real-time semi-quantitative RT-PCR.
In a parallel plate-type flow chamber, HUVECs were exposed to either steady laminar flow generating a shear stress of 25 dyne/cm2 (○) or unidirectional pulsatile laminar flow generating a shear stress of 12 ± 7 dyne/cm2 (●) for various time intervals. Static HUVECs cultured in parallel were treated with 50 ng/mL TNF-α (▪). Ratios of the relative expression levels for LKLF (A) and CYP1B1 (B) determined by semiquantitative real-time RT-PCR over static cultures were calculated and expressed as fold induction or fold repression.
