Comparison of the BrdU-labeling kinetics of potential skin emigrant DCs with the corresponding DC subtypes in skin-draining LN.

At various times after the administration of BrdU to mice, the incorporation of label into cutaneous LN DCs was determined as in Figure 4. At similar times (and usually from the same mice), the ear skin was removed, then cultured in a medium containing 6Ckine. DCs exiting over a 2-day period were collected and separated into the 2 subtypes, and the incorporation of BrdU was determined. Results for the skin emigrant DCs represent pooled data from 3 separate kinetic experiments.