CpG-ODN does not induce T-cell proliferation.

C57BL/6 mice underwent 9-day therapy with CpG-ODN or Neg-ODN. One day after the last ODN injection the draining axillary lymph nodes and spleens were harvested, stained, and analyzed via flow cytometry. Cell cycle analysis of various lymphocyte subsets was evaluated by double-staining of LN or spleen cells with specific antibodies and PI and gating on the CD8, CD4, or CD19 populations. Numbers above the gates indicate the percentage of cells with double the content of DNA, which initiated cell division.