Fig. 4.
Fetal liver hematopoiesis in rescued embryos.
(A) Analysis of E12.5 fetal liver hematopoietic cell populations. Single cell suspensions were stained with anti–c-Kit, -Ter119, -CD41, and -CD44 antibodies, and these cells (1 × 104) were analyzed by FACS. PI-negative cells were gated, and the intensity of fluorescence was measured. The ratio of cells in each quadrangle is shown. (B) Fetal liver cells were cultured for 2 days in methylcellulose medium containing various concentrations of Epo, and erythroid colonies were counted after benzidine staining. *P < .01 compared with wild-type mouse.
