CCR5 expression.

CCR5 expression during B-cell development in bone marrow as measured by RT-PCR (A), FACS (B), and QFACS (C). (A) CCR5 detection by RT-PCR of mRNA extracted from bone marrow B cells; lane 1, early-lineage B cells (pro-B and pre-B combined); lane 2, late-lineage B cells (immature B and mature B combined). no-RT indicates amplification of the PCR product not preceded by reverse transcription, to confirm that amplification from gDNA has not occurred. The results are representative of 3 experiments. (B) Surface expression of CCR5 on B-cell populations from bone marrow is shown. The shaded part of the histogram represents cells stained with anti-CCR5 antibody; the black line represents staining with the isotype control. The results are representative of 4 experiments. (C) The values represent the mean number of anti-CCR5 ABSs per cell for early lineage and late lineage bone marrow B cells. The results are representative of 3 experiments.