Effect of 8-bromo-cGMP on Ngb expression.

HN33 cells were treated with 8-bromo-cGMP (10 μM) for 2, 6, 12, or 24 hours and Western blotting was used to detect Ngb protein expression (panel A), which was quantified by computer densitometry (panel B). Quantitative RT-PCR was used to detect Ngb mRNA expression (panel C), which was quantified by computer densitometry and normalized to the expression of β-actin (panel D). Data are representative blots (panels A, C) or mean ± SEM (panels B, D) from 3 experiments. *P < .05, **P < .001 compared with 0 hours (unfilled bar).