Induction of Ngb protein expression by hemin.

HN33 cells were treated with 50 μM hemin for the indicated times (left, 0-24 hours; right, 1-3 days; C indicates control; H, hemin), and Western blotting was used to detect Ngb protein expression (panel A), which was quantified by computer densitometry (panel B). Data are representative blots (top) or mean ± SEM (bottom) from 3 experiments. *P < .05, **P < .001 compared with 0 hours (unfilled bar, left) or compared with cultures maintained for the same period without hemin (unfilled bars, right).