DC-SIGN⁺ blood cells express CD14, MHC, adhesion, and costimulatory molecules and differ from monocytes.

(A) DC-SIGN⁺ blood cells and monocytes were stained with PE-conjugated monoclonal antibodies as indicated (bold line). The thin line represents the isotype-matched control. (B) Blood monocytes were cultured in the presence of IL-4 and GM-CSF for 7 days and matured by addition of LPS for 48 hours. The expression of DC-SIGN and CD14 was analyzed at different days as indicated. Results are representative for at least 3 independent experiments.