Fig. 3.
ATRA-induced conformational changes in wild-type and I410T mutant PML/RARα fusion proteins but not in R276W mutant.
Limited trypsin digestion analysis of wild-type and mutant PML/RARα proteins. In vitro [35S]-methionine synthesized PML/RARα proteins were incubated without (−) or with (+) 1 μM ATRA, and were subsequently treated with increasing trypsin concentrations (0-25 μg/mL). Digestion products were analyzed by denaturing electrophoresis. The arrows indicate the intact PML/RARα proteins. Lower and upper asterisks indicate resistant fragments at 32 kDa and 37 kDa, respectively.
