The effect of heparin and/or IL-11 treatment on RANKL and GP130 expression in murine calvaria cells.

Calvaria cells were stimulated with either heparin alone (25 μg/mL), IL-11 alone (20 ng/mL), or heparin plus IL-11 (25 μg/mL and 20 ng/mL, respectively) as described in “Materials and methods.” Total RNA was then prepared and analyzed by Northern blot analysis for either RANKL (A) or gp130 (B).