Transcriptional activation of SIE-luciferase by STAT3 in heparin- and IL-11–treated calvaria cells.

Calvaria cells were transiently cotransfected with a 3 × SIE-luciferase reporter plasmid and a Renilla luciferase expression plasmid. The cells were then either left untreated or treated with 25 μg/mL heparin, 20 ng/mL IL-11, or a combination of heparin and IL-11 (25 μg/mL and 20 ng/mL, respectively). Twenty-four hours later the cells were lysed and luciferase activities were determined using the Dual Luciferase Reporter (DLR) Assay System as described in “Materials and methods.” Data are expressed as a mean ± SEM of 3 separate experiments. *P < .01 when luciferase activity was compared with that obtained from untreated calvaria cells. **P < .05 when luciferase activity was compared with that obtained from calvaria cells treated with IL-11 alone.