Fig. 3.
Inhibition of HSE cell proliferation by transmembrane coculture with MNCs from α-GalCer–treated mice.
Wild-type B6 mice were intraperitoneally administered vehicle (200 μL) or α-GalCer (2 μg/200 μL). At 16 hours later, isolated splenic (3 × 106) or hepatic (5 × 105) MNCs were cocultured with murine HSE (1 × 104) cells in membrane-separated wells for 72 hours. The number of HSE cells in the bottom wells was determined by crystal violet staining. Data are indicated as the mean ± SD of triplicate samples. Similar results were obtained in 3 independent experiments. *P < .01.
