Effect of neutralizing C5 on hemolysis and on granulocyte oxidative burst.

The monoclonal anti-C5 antibody 137-30 completely inhibited lysis of antibody-sensitized chicken erythrocytes in a dose-dependent manner (left panel, log scale). The same antibody reduced granulocyte oxidative burst by 73% using a final concentration of 0.7 μM (right panel; median and 20-80 percentile of 6 experiments). Granulocyte oxidative burst is given as median fluorescence intensity (MFI). ● indicates anti-C5 (clone 137-30); ○, isotype-matched control antibody (clone G3-519); ▪, whole blood incubated 10 minutes with E coli (1 × 10⁸bacteria per milliliter); and ■, baseline value (T0).