Clonality analysis using the HUMARA-PCR assay with a fluorescein-labeled primer.

Top row: DNA from granulocytes digested with RsaI. Middle row: DNA from granulocytes digested with RsaI andHpaII. Bottom row: DNA from T cells digested withRsaI and HpaII. After digestion of DNA with the restriction enzymes, the area under the curve was calculated for each allele by using Genescan software. (A) Homozygous XCIP. (B) Polyclonal XCIP. (C) Monoclonal XCIP. (D) Ambiguous XCIP.