Rapid activation of growth factor gene expression in primitive BCR-ABL–transduced +/+ and IL-3^−/− BM cells.

Sca-1⁺ lin⁻ BCR-ABL–transduced cells were sorted immediately after transduction and the cells analyzed after varying periods of culture in the presence of SF and IL-6. A semiquantitative RT-PCR was performed followed by Southern blot analysis using gene-specific cDNA probes. Each filter was then stripped and reprobed with a GAPDH probe. Lanes 1 to 5 and 6 to 10 are for MIG- and BCR-ABL–transduced +/+ cells, and lanes 11 to 15 and 16 to 20 are for the MIG- and BCR-ABL–transduced IL-3^−/− cells. Controls (CON) 1 and 2 are negative controls with no RT and water only, respectively. Results shown here are from a representative experiment.