Fig. 4.
Flow cytometric analysis of donor cells in BM and spleen 20 hours after transplantation of purified Sca-1+lin−CD62L− cells into irradiated or nonirradiated 1°ST mice.
In this representative experiment, irradiated or nonirradiated C57BL/6 mice (CD45.2+) received 1-3 × 105 sorted Sca-1+lin− CD62L− cells of B6.BoyJ origin (CD45.1+), killed 20 hours later, and donor cells in harvested BM and spleen detected by flow cytometry as described in “Materials and methods.” The frequency of donor cells is given in the upper left quadrant of each dot plot and was calculated manually using event count from listmode files containing between 5 × 105 to 1 × 106 events. The range of frequencies of detected donor cells in all experiments is given in the table.
