Percent recovery of total donor cells in BM, spleen, and PB at 1, 3, 6, and 24 hours following transplantation in irradiated or nonirradiated recipients.
Irradiated (A) or nonirradiated (B) mice received 17-90 × 106 LDBM cells, killed at the indicated time points, and analyzed for recovery of donor cells in BM, spleen, and PB as described in “Materials and methods.” Bars represent mean ± SEM recovery of total donor cells (sum of recoveries in BM + spleen + PB), and lines represent mean ± SEM recoveries in BM, spleen, or PB. Numbers at each time point represent mean recoveries. For each time point, n = 7-12 in panel A and n = 3-5 in panel B for each tissue. *P < .05 when compared with 1-hour mean of same tissue, #P < .05 when compared with spleen and PB of same time point. In order to provide an SEM value for total recovery at each time point, total recoveries were not calculated by adding the means of the recoveries from each tissue, but rather by averaging the total recoveries from each individual experiment.
