Time sequence of VE-cadherin movements on cultured HUVECs during PMN transmigration.

At time 0 seconds (B) a PMN started transmigrating through a monolayer of living HUVEC specimen that was previously immunostained with anti–VE-cadherin antibody and Alexa Fluor–conjugated secondary antibody. VE-cadherin clusters moved intermittently to create a stain-free area that corresponded to the location of PMN transmigration. Three different symbols point at the momentary moving directions of 3 VE-cadherin clusters (B). This PMN transmigration process was completed in about 120 seconds, and the resealed tricellular corner was about 10 μm left to its original location (compare panels A and F).