TF influence on TFR-2 expression in adherent cells.

HepG2 (hepatocarcinoma) and U251 (glioblastoma) were grown on coverslips, exposed to TF for 2 and 6 hours, respectively, and evaluated for TFR-2 expression. Results show the presence of discrete membrane areas in which TFR-2 is localized, more apparent in basal and in 2-hour conditions. In vitro incubation is paralleled by a constant increase in TFR-2 expression, which appears uniformly deployed on the surface after 6-hour incubation. The observed effects are dependent on the presence of HOLO-TF. APO-TF was ineffective in inducing detectable or comparable effects. TFR-2 protein was stained using G/14C2 mAb. Representative images from 5 independent experiments are shown.