Immunohistochemistry analysis of TFR-2 and TFR-1 molecules in human liver.

Three of 9 mAbs (A, G/5F8; B, G/14C2; C, G/1C11) clearly react with TFR-2 in normal formalin-fixed and paraffin-embedded liver tissue sections (original magnification, panels A-C, × 10). The reactivity is confined to hepatocytes and is strongly cytoplasmic (original magnification, panel E, × 100), whereas endothelial or bile duct cells are not stained (D, arrows; original magnification, × 40). (D,E) Images taken by using the G/5F8 mAb. TFR-1 protein displays an almost overlapping expression and localization (original magnifications, panel F, × 10; panel G, × 40). Representative data from 3 separate specimens are shown.