Reactivity of the mAbs and polyclonal antiserum against NIH-3T3/TFR-2/4B1 and control mock-transfected NIH-3T3 cells.

Primary fusion cultures were screened for binding to murine fibroblasts transfected with the human TFR-2 gene (NIH-3T3/TFR-2/4B1, white histograms), using mock-transfected NIH-3T3 cells (gray profiles) as negative control. The selection grid allowed the identification of 9 specific mAbs. A polyclonal antiserum, obtained by immunizing Balb/c mice with a mixture of 3 peptides derived from the TFR-2 sequence, displayed the same reactivity. CB26, a TFR-1–specific mAb, did not cross-react with NIH-3T3/TFR-2/4B1 cells. X-axis, fluorescence intensity/cells; y-axis, number of cells registered/channel.