Demonstration of the ability of intratumorally inoculated DCs to traffic to the spleen.

BALB/c mice with 8-day established subcutaneous Colon-26 tumors received injections of AdNK4 (A) or AdNull (C) followed by the injection of PKH26 (red fluorescence)–labeled DCs on day 11, or received injections of AdNK4 followed by the injection of PBS (B). Controls included untreated tumor-bearing mice (D). Spleens were dissected 3 days after the last treatment, and the frozen spleen sections were examined with a fluorescence microscope. In all panels, nuclei were stained with DAPI (blue fluorescence). Original magnification × 400.