Responses of splenocytes from tumor-bearing mice treated with AdNK4 and DCs.
(A) Tumor-specific CTL responses. Eight-day established subcutaneous Colon-26 tumors in BALB/c mice were treated with injection of AdNK4 (AdNK4 + DCs) or AdNull (AdNull + DCs) followed by the injection of DCs 3 days later, or treated with the injection of AdNK4 followed by the injection of PBS (AdNK4 + PBS). Controls included untreated tumor-bearing mice (no treatment). Splenocytes harvested 10 days after the last treatment were assayed for cytotoxic function against Colon-26 or BALB/3T3 target cells at an effector-target ratio of 60:1 after the in vitro restimulation. (B) Cytokine profile. Colon-26 tumor-bearing mice were treated identically to those described in panel A, and 3 × 106 spleen cells taken 10 days after the last treatment were cocultured for 5 days with 106mitomycin C–treated Colon-26 cells in 24-well culture plates. The culture medium was collected, and the levels of mouse IFN-γ and IL-4 were assayed by ELISA. For both panels, results are shown as the means ± the standard error (n = 3 per data point).
