Coimmunoprecipitation of α-defensin with PKC.

SMCs were incubated with radiolabeled α-defensin at 37°C for 30 minutes. At the end of incubation the cells were washed and lysed. Antibodies against PKC α (Anti-a), PKC β (Anti-b), or irrelevant antibodies (ir Ab) were added to the lysed cells and later precipitated by protein G conjugated to beads. In another set of experiments, the anti-LRP antibodies were added to the cells before the α-defensin and anti–α PKC antibodies (Anti-LRP-a) or anti–β PKC antibodies (Anti-LRP-b).