Binding of radiolabeled α-defensin to isolated LRP.

(A) Binding of ¹²⁵I-α-defensin to LRP-coated wells. Varying concentrations of ¹²⁵I-α-defensin were added to LRP-coated wells in the presence or absence of excess unlabeled α-defensin, and the total (■) and specific (▪) binding were measured. (B) Inhibition of α-defensin binding by rRAP and anti-LRP antibodies. ¹²⁵I-defensin (2 μM) was added to LRP-coated wells in the presence of medium alone (Cont) or medium supplemented with 20 nM rRAP (rRAP), anti-LRP antibody (anti-LRP), or irrelevant antibody (irAntib).