The reversibility of the inhibitory effect of α-defensin.

Aortic rings were incubated with (▪) or without (▴) 1 μM α-defensin for 30 minutes. After washing, the response to PE was determined immediately (0 minute) or after 15, 30, 60, 75, or 120 minutes. The EC₅₀ was determined as described in “Materials and methods.”