Fig. 5.
![Fig. 5. Immunization of mice with stressed apoptotic 12B1-D1 cells induces IFN-γ, IL-2 secretion by splenocytes and T-cell proliferation. / Heat-stressed or nonstressed 12B1-D1 cells (2 × 106) were treated with 40 nM AP20187 for 6 hours and then injected to BALB/c mice subcutaneously. Splenocytes from immunized mice were harvested 5 days later and restimulated with mitomycin C–treated 12B1-D1 cells. (A) CTLL-2 bioassay was used to determine the IL-2 production. (B) IFN-γ secretion was determined by ELISPOT. (C) T-cell proliferation was determined by [3H]thymidine incorporation. Representative data from 1 of 3 experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/12/10.1182_blood-2002-05-1389/5/h82323488005.jpeg?Expires=1724226862&Signature=zmpQKc-XQeXgO6Y2kRfJmALTxlNhteWBBZdnshpCFDOsEjISe-J2C1TepqyuOMOLTp5SixyzR0AXMSpxSL~a--~iCsPeVA9~V3SNWaG431oXNGlIbmLOOF4cSX2mnWPNvZsiTTJvG91Y2aoti8EXwvSfAIov~187BR1M7inM51pPc8UN5sBK8x7HdcTMlDT3IPQrvKiDL2eVaFwTU8cNiQ99dQVY2YEuX13zJDj6zY5HYF2K9M7YjnAleDTi2qeMyt~9tKc5v4fJ1nmdQYgi4Do3OfpkyRjya9JBc1V5GtE8Xwi97eaTEHPnJUnXNJoYUZG84GDKPUidRoZBB2XKEw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunization of mice with stressed apoptotic 12B1-D1 cells induces IFN-γ, IL-2 secretion by splenocytes and T-cell proliferation.
Heat-stressed or nonstressed 12B1-D1 cells (2 × 106) were treated with 40 nM AP20187 for 6 hours and then injected to BALB/c mice subcutaneously. Splenocytes from immunized mice were harvested 5 days later and restimulated with mitomycin C–treated 12B1-D1 cells. (A) CTLL-2 bioassay was used to determine the IL-2 production. (B) IFN-γ secretion was determined by ELISPOT. (C) T-cell proliferation was determined by [3H]thymidine incorporation. Representative data from 1 of 3 experiments are shown.
