GM-CSF and G-CSF enhance TLR2 and CD14 expression on neutrophils.

Neutrophils were incubated with GM-CSF (100 U/mL), G-CSF (100 U/mL), or medium alone for 2 hours prior to staining and FACS analysis. Isotype-control Ab–stained cells are shown as gray histograms. Specific mAb–stained cells are shown as black line histograms. Geometric mean fluorescence intensity (SD). Neutrophils treated with medium alone: isotype control, 7.9 (0.4); TLR2, 12.3 (0.3); TLR4, 8.5 (0.3); and CD14, 17.5 (0.3). Neutrophils treated with GM-CSF: isotype control, 7.9 (0.4); TLR2, 19.4 (0.4); TLR4, 9.2 (0.4); and CD14, 33.8 (0.4). Neutrophils treated with G-CSF: isotype control, 7.9 (0.4); TLR2, 18.0 (0.4); TLR4, 8.8 (0.3); and CD14, 24.7 (0.4). Significance of difference between treated cells and untreated controls: **P < .001 by Probability Binning ChiT analysis (ChiT(X) > 450); P < .001 by Kolmogorov-Smirnoff analysis.