Regulation of C/EBPε, c-myc, and c-myb in 32D/FLT3 and 32D/FLT3/ITD cells.
32D/FLT3 cells were washed and transferred from medium containing IL-3 to medium containing G-CSF (20 ng/mL) without (lanes 1-6) or with (lanes 7-12) FL (100 ng/mL) and cultured for 9 days. 32D/FLT3/ITD cells were treated without (lanes 13-19) or with (lanes 20-26) CEP-701 (5 nM) in the presence of G-CSF (20 ng/mL) for 11 days. Every other day the medium was replaced. Total cellular RNA was extracted from 1 × 107 32D/FLT3 cells on days 0, 1, 3, 5, 7, and 9. Total cellular RNA was also prepared from 1 × 107 32D/FLT3/ITD cells on days 0, 1, 3, 5, 7, 9, and 11. RNA (15 μg from each sample) was then subjected to Northern blotting with C/EBPε, c-myc, c-myb, and actin cDNA probes.
