CEP-701 overcomes the FLT3/ITD-mediated block of differentiation in 32D/FLT3/ITD cells.
32D/FLT3/ITD cells were treated with or without CEP-701 (5 nM) in the presence of G-CSF (20 ng/mL) for 11 days. Every other day, fresh medium containing G-CSF with or without CEP-701 was added to cells to replace the old medium. (A) Cytospins were prepared on days 0 and 11. The morphologic features of the cells were visualized by means of Wright-Giemsa staining followed by light microscopy. Original magnification, × 100. (B) Total cellular RNA was prepared from 1 × 107 cells on days 0, 1, 3, 5, 7, 9, and 11 of culture without (lanes 1-7) or with (lanes 8-14) CEP-701. RNA (15 μg from each sample) was then subjected to Northern blotting with MPO, lysozyme, and actin cDNA probes.
