American Society of Hematology

Fig. 7.

$Fig. 7. Activation of p53 is independent of Bax conformational changes. / (A) Flow cytometric analysis of conformational changes of Bax and p53 activation in cells from a representative CLL patient incubated with medium alone or in the presence of 5 μg/mL fludarabine, 10 μM dexamethasone, or the FCM combination. (B) Western blot analysis of p53 protein. Whole-cell lysates were obtained from 2 × 106 cells from a representative CLL patient incubated with medium alone (C), fludarabine (F), FCM, or dexamethasone (D), in the presence (+) or absence (−) of 200 μM Z-VAD.fmk for 24 hours, and were analyzed by Western blot. (C) Cytosolic and mitochondrial fractions were obtained from 50 × 106cells incubated in the absence (C) or presence of the FCM combination for 24 hours. Mitochondrial fractions were either left untreated or treated with alkali. All fractions were analyzed by Western blot.$

Activation of p53 is independent of Bax conformational changes.

(A) Flow cytometric analysis of conformational changes of Bax and p53 activation in cells from a representative CLL patient incubated with medium alone or in the presence of 5 μg/mL fludarabine, 10 μM dexamethasone, or the FCM combination. (B) Western blot analysis of p53 protein. Whole-cell lysates were obtained from 2 × 10⁶ cells from a representative CLL patient incubated with medium alone (C), fludarabine (F), FCM, or dexamethasone (D), in the presence (+) or absence (−) of 200 μM Z-VAD.fmk for 24 hours, and were analyzed by Western blot. (C) Cytosolic and mitochondrial fractions were obtained from 50 × 10⁶cells incubated in the absence (C) or presence of the FCM combination for 24 hours. Mitochondrial fractions were either left untreated or treated with alkali. All fractions were analyzed by Western blot.

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