Cellular localization of Bax and cytochrome c during drug-induced apoptosis.

Cells from a representative patient were incubated in the absence or presence of the FCM combination for 24 hours. Cells were immunostained with anti-Bax (clone YHT-6A7, green fluorescence) and anti–cytochrome c (red fluorescence). Photographs of the same fields were obtained with optical filters specific for green and red light (original magnifications, × 600). Arrows indicate the same apoptotic cell showing a punctuated Bax staining and having lost cytochrome c. Asterisks on the lower panel indicate viable cells (negative staining for Bax and bright punctuated signal for cytochrome c).