Fig. 1.
Fig. 1. Drug-induced apoptosis is accompanied by conformational changes in Bax. / (A) Cells from a representative CLL patient were incubated in the presence or absence of 5 μg/mL fludarabine, 10 μM dexamethasone, or the FCM combination for 24 hours. Bax conformational changes were determined by staining with anti-Bax (clone YHT-6A7) and flow cytometric analysis. Cell viability was quantified by annexin V binding. In FCM-treated cells, a shift in the signal of fluorescence 2 (585 nm) and 3 (630 nm) was observed owing to the incorporation of mitoxantrone. The percentage of positive cells is indicated in each panel. (B) Correlation between cell viability and Bax-positive cells following incubation with medium alone, fludarabine, dexamethasone, or the FCM combination in cells from 33 CLL patients.

Drug-induced apoptosis is accompanied by conformational changes in Bax.

(A) Cells from a representative CLL patient were incubated in the presence or absence of 5 μg/mL fludarabine, 10 μM dexamethasone, or the FCM combination for 24 hours. Bax conformational changes were determined by staining with anti-Bax (clone YHT-6A7) and flow cytometric analysis. Cell viability was quantified by annexin V binding. In FCM-treated cells, a shift in the signal of fluorescence 2 (585 nm) and 3 (630 nm) was observed owing to the incorporation of mitoxantrone. The percentage of positive cells is indicated in each panel. (B) Correlation between cell viability and Bax-positive cells following incubation with medium alone, fludarabine, dexamethasone, or the FCM combination in cells from 33 CLL patients.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal