Determination of the affinity of MIP1α (BB10010) for HS from different cell types.

Intact ³H-labeled HS from human bone marrow stroma (A), bovine aortic endothelial cells (B), and murine fibroblast cells (C) were applied to an MIP1α (BB10010) Affi-Gel column (•) in 0.15 M NaCl. The same types of HS were also applied to a control column (●). Bound material was eluted with a stepwise NaCl gradient (^{. . . . . . .}). Graphs are representative of duplicate experiments.