Comparison of the relative affinity of HS for monomeric, dimeric, and tetrameric MIP1α aggregation mutants.

Intact murine fibroblast ³H-radiolabeled HS was applied to an Affi-Gel column, prepared with the monomeric (■), dimeric (▪), or tetrameric (▨) MIP1α aggregation mutants, in 0.15 M NaCl. Bound material was eluted with the NaCl concentrations indicated. Results show the averages from 6 experiments. Error bars of the percentage HS eluted are depicted.