Analysis of normal peripheral blood DCs for the expression of CD52 and either CD11c or CD123.

PBMCs from a healthy donor were stained for lineage markers (CD3, CD14, CD16, CD19, CD20, CD34, and CD56), HLA-DR, CD52, and either CD11c (A) or CD123 (B). The majority of CD11c-expressing lin⁻HLA-DR⁺ DCs also express CD52, whereas CD123⁺ DCs varied in their expression of CD52. The result shown is representative of 3 independent experiments. ECD indicates energy-coupled dye (ECD-Streptavidin; Immunotech, Beckman Coulter).