Recovery from myelosuppression in wild-type and STAT5-deficient chimeric mice following a single injection of 5-fluorouracil.

Chimeric mice were generated by transplantation of wild-type or STAT5ab^−/− BM cells into lethally irradiated recipient HW80 mice. Reconstitution of irradiated recipients was determined by hemoglobin electrophoresis. After reconstitution, mice were injected once with 150 mg/kg 5 fluorouracil (5-FU). One week prior to the 5-FU injection and at the indicated times after injection, the mice were bled and the hematocrit was determined. A second experiment was also performed with fewer of the intermediate time points (data not shown). At all time points hemoglobin electrophoresis was also performed to check for donor engraftment (data not shown).