Fig. 2.
Localized expression of furin convertase in differentiated Dami cells.
Immunofluorescence was performed using Dami cells undifferentiated or differentiated for 3 days with 10−7 M PMA. Cells were fixed, permeabilized, and stained with (A) mouse anti–GATA-1 (1/100) or rabbit anti-furin (1/1000) and then visualized by fluorescence microscopy, using a secondary antibody coupled to fluorescein isothiocyanate. (B) Three-day differentiated cells were costained with rabbit anti-furin (FITC) and mouse anti-58K (1/100; rhodamine). Fluorescence colocalization corresponds to Golgi structures around the nuclei. Preimmune serum was used as negative control. Magnification, × 400.
