Expression of hFIX in rhesus macaques after liver-targeted delivery of rAAV CAGG-FIX.

(A) ELISA assay. The hFIX concentration in rhesus plasma was determined at the indicated times after liver-directed administration of rAAV CAGG-FIX in 5 rhesus macaques (M1, ■; M2, - -⋄- -; M3, ▿; M4, ♦; and M5, ○). Samples from monkeys 1 to 4 were evaluated independently on 4 occasions; those from monkey 5 were assessed on 2 separate occasions. Results are means ± SEM. (B) Western blot analysis of barium citrate precipitated rhesus serum samples.