Intraepithelial lymphocyte development after gene transfer.

CD4 and CD8 immunohistochemical stainings (n = 3) were used to phenotype gut-specific intraepithelial lymphocytes (IEL) from frozen intestine sections of RAG-2−/−, C57BL/6, and RAG-2–transduced mice (6 months after transplantation). CD4⁺ and CD8⁺IELs were detected in RAG-2–transduced mice as well as in wild-type C57BL/6 controls. Original magnifications × 200.