Viable (7-AAD⁻) Lin⁻FGFR⁺ cells coexpress both primitive hematopoietic (AC133) and endothelial-specific (VE-cadherin) cell surface markers.

CD34⁺-enriched cells were isolated from lineage-depleted mobilized peripheral blood and gated to exclude debris with low FSC and cells with high SSC (R1) and nonviable (7-AAD⁺) cells (R2). (A) R3 defines viable Lin⁻FGFR⁺cells. Most of the viable Lin⁻FGFR⁺ cells are shown to have low SSC. (B) Boolean gating is used to show coexpression of both AC133 and VE-cadherin by viable Lin⁻FGFR⁺ cells. These plots are representative of 2 separate experiments.