Flow cytometry of the CD34⁺FGFR⁺ population.

(A) Forward and side scatter analysis of CD34⁺-enriched cells isolated from mobilized peripheral blood. R1 defines a region that excludes debris with low FSC and cells with high SSC. (B) R2 defines viable (7-AAD⁻) cells. (C) R3 defines viable CD34⁺FGFR⁺ cells. (D) Boolean gating is used to illustrate the FSC/SSC characteristics of viable CD34⁺FGFR⁺ cells and shows that most of these cells are small (low FSC) and display little granularity (low SSC).