Flow cytometric profiles of CD59 expression on peripheral blood granulocytes and bone marrow CD34⁺ cells from 3 PNH patients and a healthy volunteer.

In each histogram, thick and thin lines in panels A-D or solid and dashed lines in panels E-H show reacted samples and negative controls, respectively. Horizontal and vertical axes are fluorescence intensity of CD59 (FL1) and cell counts, respectively. (A) Granulocytes from a healthy volunteer. (B) Granulocytes from case 1. (C) Granulocytes from case 2. (D) Granulocytes from case 3. (E) CD34⁺ cells from a healthy volunteer. (F) CD34⁺cells from case 1. (G) CD34⁺ cells from case 2. (H) CD34⁺ cells from case 3.