Isolation with a fluorescence-activated cell sorter of each population expressing CD59 among CD34⁺ cells and granulocytes.

Bone marrow MNCs were stained with monoclonal antibodies to CD59, CD34, and CD45, divided into CD34⁺CD59⁻ and CD34⁺CD59⁺ populations according to the gates shown by rectangles in panels A to C (A, case 1; B, case 2; C, case 3), and sorted. Vertical and horizontal axes are the fluorescence intensities of CD34 and CD59, respectively. Peripheral blood granulocytes were stained with a monoclonal antibody to CD59, divided into CD59⁻ CD59^+/−, and CD59⁺populations according to the gates shown by rectangles in panels D to F (D, case 1; E, case 2; F, case 3), and sorted. Vertical and horizontal axes are the side scatter and fluorescence intensity of CD59, respectively.