Effect of a dominant-negative Akt mutant on BCR/ABL-dependent VEGF mRNA expression.

Ton.B210-X cells induced to express BCR/ABL were transfected with the dominant-negative Akt-K179M mutant or a control plasmid (Control) and were subjected to Northern blotting. The blot was probed for VEGF and reprobed for β-actin. Densitometric evaluation of VEGF mRNA expression is shown below the blot analyses.