Effect of pharmacologic inhibitors on Ras-G12V–induced VEGF reporter gene activity.

Ton.B210-X cells were transfected with the oncogenic Ras-G12V mutant or a control plasmid (together with VEGF-Luc/pCMV-βGal) and incubated with PD98059 (25 μM), LY294002 (20 μM), or control medium. After 48 hours, cells were harvested and assayed for luciferase and βGal activities. VEGF promoter activity was reported as the ratio VEGF-Luc/pCMV-βGal and was expressed as a percentage of control (transfection with control plasmid; Control). Results represent the means ± SD of 3 independent experiments.