Growth kinetics and induction of VEGF in Ton.B210-X cells.

(A) Ton.B210-X cells were grown in IL-3 for 3 days, starved of IL-3 for another 3 days, and then induced to express BCR/ABL by the addition of doxycycline. (B) Ton.B210-X cells were starved for 48 hours and were maintained under hypoxic conditions (starved + hypoxia) or normal atmosphere (starved) overnight. Total RNA was extracted and subjected to Northern blot analysis using a murine VEGF probe. The β-actin loading control and densitometric evaluation of VEGF mRNA expression relative to β-actin mRNA expression (relative mRNA expression) is shown below the blot analyses.