![Fig. 2. Reovirus replication in lymphoma cell lines in vitro. / (A) Reovirus protein synthesis in infected Burkitt lymphoma and diffuse large B-cell NHL cell lines. Established human Burkitt lymphoma (Raji, CA46, Daudi, Ramos, and ST486) and the diffuse large B-cell NHL (OCY-LY1, OCY-LY2, OCY-LY8, and OCY-LY10) cell lines were infected with reovirus. The cells were pulse-labeled with [35S]-methionine for 6 hours at 66 and 90 hours after infection for the Burkitt lymphomas and diffuse large B-cell lymphomas, respectively. Following labeling, the cells were harvested and lysed, and reovirus proteins were immunoprecipitated from part of the lysate by means of rabbit polyclonal antireovirus antibodies. Immunoprecipitates were then analyzed by SDS-PAGE. Reovirus protein synthesis is apparent in all diffuse large B-cell lymphomas as well as in the Raji and CA46 cell lines, but not the Daudi, Ramos, or ST486 cell lines. The 3 size classes of reovirus proteins (λ, μ, and ς) are indicated on the left. (B) Viral progeny. Established Burkitt lymphoma and diffuse large B-cell NHL cell lines were infected with reovirus. At 0 and 96 hours after infection, the cells were harvested and freeze-thawed 3 times, and the virus titer in the lysate was determined by plaque assay on L929 cells. Error bars indicate standard deviations of the means from 3 separate wells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/12/10.1182_blood-2002-02-0503/5/h82323490002.jpeg?Expires=1724259903&Signature=bBZZa5-1VXhevqQwqu9gyLMf5NERPO4O36i~NqQ9Heki99NF0IeZoYyeiQjz4nYyG6p3hQe681He7UjnZnPsQWLo4Gbgw~Kq4O~2J7~Sn2~fYgvH2DTrS03A43U63EfYwMUSGszfPA1BVUR4v41M~aMsUHebLCFrji5Kd8nc2ZvY8qohcuP8xV-U9fRBxWyAcV74oRNMNChu8TDsZLhiLSeWy~uzggjJvc~fqYPTbS76vI7cWId6Sa6pwoKb-nD06YPPMWTwk8HDYDBxYWgtH-b5drmS1MGZR3ry3w~fg9VUPtvAhJA0ImfOWRA0jhckwl7KO9SNlCGDjiptotcFiQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Reovirus replication in lymphoma cell lines in vitro.
(A) Reovirus protein synthesis in infected Burkitt lymphoma and diffuse large B-cell NHL cell lines. Established human Burkitt lymphoma (Raji, CA46, Daudi, Ramos, and ST486) and the diffuse large B-cell NHL (OCY-LY1, OCY-LY2, OCY-LY8, and OCY-LY10) cell lines were infected with reovirus. The cells were pulse-labeled with [35S]-methionine for 6 hours at 66 and 90 hours after infection for the Burkitt lymphomas and diffuse large B-cell lymphomas, respectively. Following labeling, the cells were harvested and lysed, and reovirus proteins were immunoprecipitated from part of the lysate by means of rabbit polyclonal antireovirus antibodies. Immunoprecipitates were then analyzed by SDS-PAGE. Reovirus protein synthesis is apparent in all diffuse large B-cell lymphomas as well as in the Raji and CA46 cell lines, but not the Daudi, Ramos, or ST486 cell lines. The 3 size classes of reovirus proteins (λ, μ, and ς) are indicated on the left. (B) Viral progeny. Established Burkitt lymphoma and diffuse large B-cell NHL cell lines were infected with reovirus. At 0 and 96 hours after infection, the cells were harvested and freeze-thawed 3 times, and the virus titer in the lysate was determined by plaque assay on L929 cells. Error bars indicate standard deviations of the means from 3 separate wells.
