Effect of reovirus on lymphoma cell lines in vitro.

(A) Reovirus-induced cytopathic effects in various lymphoma cell lines in vitro. Established human Burkitt lymphoma (Raji, CA46, Daudi, Ramos, and ST486) and diffuse large B-cell NHL (OCY-LY1, OCY-LY2, OCY-LY8, and OCY-LY10) cell lines were exposed to reovirus at an MOI of 20 PFUs per cell, and photomicrographs were taken at 96 hours after infection. Cytopathic effects are apparent in all diffuse large B-cell NHL as well as the Raji and CA46 cell lines, but not in Daudi, Ramos, or ST486 cells. Original magnification × 200. (B) Remaining viability. Viability of the same cell lines at 96 hours after infection was assessed by staining the cells with 0.25% trypan blue; unstained (viable) cells from 3 independent wells were counted by means of a hemocytometer. As expected, there is good correlation between cell death and manifestation of the cytopathic effects shown in Figure 1A. ■ indicates control; and ░, reovirus.