Comparison of relative binding avidities of cross-linked CD3 antibodies.

(■, BM), bivalent^Cys444 monomer; (▵, BD), bivalent^Cys444 dimer; (○, MM), monovalent^Cys444 monomer; (×, MD), monovalent^Cys444 dimer; (+, BM^WT), bivalent^WT monomer; (⋄, alem), CD52 mAb alemtuzumab. Fluorescence values obtained in the absence of competitor mAb (♦, 100% fluorescence), or in the absence of competitor mAb and biotinylated CD3 mAb (▪, 0% fluorescence) are indicated. Each point is the mean of duplicate tests.